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瀹為獙瀹よ祫璁綉

鏃ユ湰绉戝瀹跺缓绔嬫柊寮廝CR鎵╁鏂规硶锛岃兘鍦ㄥ父娓?7鈩冨揩閫熸墿澧濪NA

瀹為獙瀹よ祫璁綉鏃堕棿:2019-07-09 鐐瑰嚮: 娆?/span> 鐧惧害鎼滅储

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鏃ユ湰绉戝瀹跺缓绔嬫柊寮廝CR鎵╁鏂规硶锛岃兘鍦ㄥ父娓?7鈩冨揩閫熸墿澧濪NA

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鏃ユ湰绉戝瀹跺缓绔嬫柊寮廝CR鎵╁鏂规硶锛岃兘鍦ㄥ父娓?7鈩冨揩閫熸墿澧濪NA

璇ラ」琚О涓篖-TEAM锛圠ow-TEmperature AMplification锛夌殑鏂规硶鍘嗙粡绉戝瀹朵簲骞寸殑鐮旂┒锛岀浉姣斾紶缁熺殑PCR鏈夊椤逛紭鍔裤€?/p>

鍑€熷叾渚垮埄鐨?ldquo;涓€姝ユ硶”璁捐锛孡-TEAM鏈夋晥閬垮厤浜嗛珮娓╁彉鎬у拰閫€鐏殑姝ラ锛屼篃鏃犻渶鍊熷姪甯歌PCR鎵€闇€瑕佺殑鐗瑰畾浠櫒銆?/span>杩欑楂樻晥浣庢垚鏈殑鏂规硶鑳藉闃叉铔嬬櫧璐ㄥ彉鎬э紝鍥犳瀵规椿浣撶粏鑳炲疄鏃跺垎鏋愭彁渚涗簡涓€绉嶆柊鐨勮矾寰勩€?/p>

鍦ㄥ彂琛ㄤ簬銆婃湁鏈轰笌鐢熺墿鍒嗗瓙鍖栧銆嬶紙Organic & Biomolecular Chemistry锛夋潅蹇楋紝棰樹负“Leak-free million-fold DNA amplification with locked nucleic acid and targeted hybridization in one pot”鐨勭爺绌朵腑锛岀爺绌惰€呭皢涓€绉嶅悕涓?span style="font-weight: 700; border: 0px; margin: 0px; padding: 0px;">閿佹牳閰革紙locked nucleic acids锛孡NAs锛?/span>鐨勫悎鎴愬垎瀛愬紩鍏ュ埌浜咲NA閾撅紝鑰岃繖绉嶅悎鎴愬垎瀛愯兘澶熻DNA鍦ㄦ潅浜よ繃绋嬩腑鍙樺緱鏇村姞绋冲畾銆?/p>

鍙﹀锛屽姞鍏NA浠ュ悗杩樺甫鏉ヤ簡鍙︿竴绉嶆剰澶栦絾鏈夌泭鐨勭粨鏋溿€傜爺绌跺洟闃熻瀵熷埌DNA闈炵壒寮傛€ф墿澧炵殑鎯呭喌锛堟敞锛氬師鏂囨槸leak DNA amplification锛屽鏈夋洿鎭板綋涓枃琛ㄨ堪锛屾杩庢枃鏈暀瑷€锛夋湁鎵€鍑忓皯锛岃€岃繖鏄疍NA鎵╁鐮旂┒涓暱鏈熷洶鎵扮瀛﹀鐨勪竴涓棶棰樸€?span style="font-weight: 700; border: 0px; margin: 0px; padding: 0px;">杩欏氨鎰忓懗鐫€锛孡NA鑳藉鍑忓皯鐤剧梾璇婃柇涓殑閿欒涔熷氨鏄亣闃虫€ф儏鍐电殑鍑虹幇銆?/span>

鏃ユ湰绉戝瀹跺缓绔嬫柊寮廝CR鎵╁鏂规硶锛岃兘鍦ㄥ父娓?7鈩冨揩閫熸墿澧濪NA

涓滀含宸ヤ笟澶у鍓暀鎺圞en Komiya琛ㄧず锛?ldquo;鍙戠幇LNA鍦ㄥ厠鏈岲NA鎵╁鍙嶅簲涓父瑙佺殑闈炵壒寮傛€ф墿澧為棶棰樹腑鐨勬柊浣滅敤锛屾垜浠緢鎰忓銆傛垜浠鍒掕繘涓€姝ヨ缁嗘帰绌堕潪鐗瑰紓鎬ф墿澧炶儗鍚庣殑鏈哄埗锛屽苟杩涗竴姝ユ敼鍠凩-TEAM鎶€鏈殑鐏垫晱搴﹀拰閫熷害銆?rdquo;

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鍙傝€冭祫鏂欙細

1.Leak-free million-fold DNA amplification with locked nucleic acid and targeted hybridization in one pot

2.A rapid, easy-to-use DNA amplification method at 37 Celcius

锛堟湰鏂囨潵婧愶細鍖诲涓枃缃?锛?

(璐d换缂栬緫锛氬瓙璞?

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